LOCUS Exported 4081 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4081)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4081
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 435..1322
/label=2X35S promoter + TL enhancer
misc_feature 1323..1523
/label=nEYFP
misc_feature 1524..1589
/label=MCS
/note="multiple cloning site"
misc_feature 1602..1812
/label=35S terminator
rep_origin complement(2262..2850)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3021..3881)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3882..3986)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
1321 ccatgggcgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
1381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
1501 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
1561 cagtcgacgg taccgcgggc ccgggatcca cctgatctag agtccgcaaa aatcaccagt
1621 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca
1681 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
1741 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
1801 aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca gtaatcatgg
1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
1981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
3301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
4021 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
4081 c
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