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  • 产品名称：Cysteinyl Leukotriene Receptor 2 (CYSLTR2), ELISA Kit

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简单介绍：
Cysteinyl Leukotriene Receptor 2 (CYSLTR2), ELISA Kit
详情介绍：
Product Name

Cysteinyl Leukotriene Receptor 2 (CYSLTR2), ELISA Kit

Full Product Name

Human Cysteinyl Leukotriene Receptor 2 (CYSLTR2) ELISA Kit

Product Synonym Names
CYSLT2; GPCR; CYSLT2R; HG57; HPN321; HGPCR21; G-protein coupled receptor GPCR21; G-protein coupled receptor HG57
Product Gene Name

CYSLTR2 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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OMIM
AB038269 mRNA
3D Structure
ModBase 3D Structure for Q9NS75
Species Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CYSLTR2. No significant cross-reactivity or interference between CYSLTR2 and analogues was observed.
Samples
Tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.108ng ml.<="" div="">
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYSLTR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%< div="">
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYSLTR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%< div="">
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYSLTR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CYSLTR2 purchase
MBS456849 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cysteinyl Leukotriene Receptor 2 (CYSLTR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYSLTR2. The ELISA analytical biochemical technique of the MBS456849 kit is based on CYSLTR2 antibody-CYSLTR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYSLTR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYSLTR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
CYSLTR2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYSLTR2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYSLTR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYSLTR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYSLTR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYSLTR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Typical Testing Data/Standard Curve (for reference only) of CYSLTR2 elisa kit
CYSLTR2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for CYSLTR2. It may not necessarily be applicable to this product.
NCBI GI #
818213437
NCBI GeneID
57105
NCBI Accession #
NP_001295394.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001308465.2 [Other Products]
UniProt Primary Accession #
Q9NS75 [Other Products]
UniProt Secondary Accession #
Q9HCQ2[Other Products]
UniProt Related Accession #
Q9NS75[Other Products]
Molecular Weight
39,635 Da
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NCBI Official Full Name
cysteinyl leukotriene receptor 2
NCBI Official Synonym Full Names
cysteinyl leukotriene receptor 2
NCBI Official Symbol
CYSLTR2  [Similar Products]
NCBI Official Synonym Symbols
HG57; CYSLT2; GPCR21; HPN321; CYSLT2R; KPG_011; hGPCR21; PSEC0146
  [Similar Products]
NCBI Protein Information
cysteinyl leukotriene receptor 2
UniProt Protein Name
Cysteinyl leukotriene receptor 2
UniProt Synonym Protein Names
G-protein coupled receptor GPCR21; hGPCR21; G-protein coupled receptor HG57; HPN321
Protein Family
Cysteinyl leukotriene receptor
UniProt Gene Name
CYSLTR2  [Similar Products]
UniProt Synonym Gene Names
CYSLT2; CYSLT2R; CysLTR2; hGPCR21  [Similar Products]
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NCBI Summary for CYSLTR2
The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR1. This encoded receptor is a member of the superfamily of G protein-coupled receptors. It seems to play a major role in endocrine and cardiovascular systems. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYSLTR2
CYSLTR2: Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol- calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: cysteinyl leukotriene receptor activity; leukotriene receptor activity; peptide receptor activity, G-protein coupled; protein binding

Biological Process: immune response; neuropeptide signaling pathway; phospholipase C-activating G-protein coupled receptor signaling pathway; positive regulation of angiogenesis
Research Articles on CYSLTR2
1. The expression of CysLTR1 and CysLTR2 is higher in the lymphocytes of hyperplasic tonsils in nonallergic children
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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