产品详情
简单介绍：
Immortalized-Human-Urethral-Epithelial-Cells
详情介绍：
| Print Version | |
| BioSafety Level | II |
| Organism | Homo sapiens |
| Source Organ | Membranous urethral tissue explants |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Recommended Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Applications | For Research Use Only |
| Immortalization Method | Serial passaging and transduction with recombinant retrovirus vector pLXSN containing the HPV16 E6E7 oncogenes |
| Description | Primary human urethral epithelial cells were isolated and immortalized with the recombinant retrovirus vector pLXSN containing the HPV16 E6E7 oncogenes to achieve the generation of the Immortalized Human Urethral Epithelial Cells. The cells have telometric activity, and expresses cytokeratins 13 and 16 markers and the ASGP-R (asialoglycoprotein) receptor. The immortalized cells retained the primary phenotypes when compared to the primary cells. Immortalized Human Urethral Epithelial Cells are an unlimited source of tissue culture urethral epithelial cells for downstream applications. It may be used to study the urethral epithelium and possible investigations in pathogenesis in the urethra. |
| Procedure Overview |  |
| Image |  |
| Propagation | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow X Series Medium available from abm (TM0629). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Quality Control | Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. |
| Reference | Harvey et al. (2002) Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection. Infect Immun. 70(10): 5808-5815. |
| Disclaimer | 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
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