产品详情
简单介绍：
OCT4 Purified Protein
详情介绍：
| Print Version | |
| Organism | Human |
| Accession # | Q01860 |
| Alias | POU5F1, OCT3, OTF3 |
| Protein Type | Wild Type |
| Protein Sequence | HHHHHHAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSNESGGGGSPGRRRRRRRRR |
| Protein Family | Belongs to the POU transcription factor family. Class-5 subfamily. |
| Functions | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). OCT4 forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. OCT4 is critical for early embryogenesis and for embryonic stem cell pluripotency. OCT4 is one of the four factors in both the Thomson and the Yamanaka protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration. |
| Cell Types | Expressed in developing brain. |
| Protein Location | Nucleus. |
| Tag | 6X His tag at N-terminus and 9X Poly-Arginine at C-terminus |
| Form | Liquid |
| Expression System | E.coli |
| Type | Purified Protein |
| Purification | Affinity Chromatography |
| Quality Control | By Western Blot Analysis |
| Concentration | 250ug/ml |
| Storage | PBS |
| Shipping | On dry ice. |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
| References | Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. |
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