产品详情
简单介绍:
Piccolo - 142 113
详情介绍:
Cat. No. 142 113 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not recommended ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 (see remarks) gallery IHC-P/FFPE: 1 : 500 gallery |
Immunogen | Recombinant protein corresponding to AA 2012 to 2351 from rat Piccolo (UniProt Id: Q9JKS6) |
Epitop |
Epitop: AA 2011 to 2350 from rat Piccolo (UniProt Id: Q9JKS6) |
Reactivity |
Reacts with: rat (Q9JKS6), mouse (Q9QYX7). Other species not tested yet. |
Specificity | Specific for piccolo. |
Remarks |
WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. IHC: This antibody requires mild fixation. recommended protocol |
Datasheet | 142_113.pdf |
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