产品详情
简单介绍:
Abeta 42 - 218 703
详情介绍:
Cat. No. 218 703 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 1000 (ECL detection) (see remarks) gallery IP: not tested yet ICC: 1 : 500 IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P/FFPE: 1 : 100 (see remarks) gallery ELISA: yes suitable only as capture antibody, cat. no. 218 211 is recommended detector antibody for gallery (protocol) |
Immunogen | Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067) |
Reactivity |
Reacts with: human (P05067), rat (P08592), mouse (P12023). Other species not tested yet. |
Specificity | Specific for Abeta 42 with weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests. |
Remarks |
WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min. IHC: Formic acid treatment required recommended protocol. IHC-P: Formic acid treatment required. |
Datasheet | 218_703.pdf |
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