产品详情
简单介绍:
Glu-α-tubulin - 302 011
详情介绍:
Cat. No. 302 011 | 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 200 gallery IHC: 1 : 200 gallery IHC-P/FFPE: yes ELISA: yes |
Clone | 1D5 |
Subtype | IgG1 (κ light chain) |
Immunogen | Synthetic peptide corresponding to AA 437 to 450 from human Glu-α-tubulin |
Epitop | Epitop: AA 448 to 450 from human Glu-α-tubulin |
Reactivity |
Reacts with: human, rat, mouse, zebrafish, eukaryotes. Other species not tested yet. Detects also cilia of Paramecium. |
Specificity | Specific for detyrosinated α-tubulin (glu-tubulin) and polyglutamylated tubulin (also β-tubulin). No cross reaction to tyrosinated tubulin. |
Remarks |
WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal. |
Datasheet | 302_011.pdf |
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