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产品介绍 |
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| 品牌 |
PBL Assay Science |
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| 货号 |
11002-1 |
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| 品名 |
Human IFN Alpha Sampler Set |
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| 规格 |
1 x 105 units |
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| 分子量 |
19.2 -19.7 kDa |
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| 来源 |
cDNA of interferons were obtained from human leukocyte mRNA, and expressed in E.coli |
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| 纯度 |
> 95% by SDS-PAGE stained by Coomassie Blue |
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| 生物活性 |
Activities of the interferons are measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV) |
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| 缓冲液 |
Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
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| 内毒素 |
< 1 EU/μg |
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| 纯度 |
≥ 95% |
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|
纯化方法 |
Purified from E. coli by a combination of ion exchange, hydrophobic interaction and size exclusion chromatography |
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| 贮存 |
For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles |
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- 包含12 种不同的人类 IFN α 亚型，用于差异活性研究
- 从人白细胞mRNA中获得干扰素的cDNA，并在大肠杆菌中表达
- 提供 100,000 个单位的 IFNa-A、IFNa-B2、IFNa-C、IFNa-D (1[Val 114 ])、IFNa-F、IFNa-G、IFNa-H2、IFNa-I、IFNa-J1、IFNa-K , IFNa-4b, and IFNa-WA (对应于 IFNa-2a, IFNa-8, IFNa-10, IFNa-1, IFNa-21, IFNa-5, IFNa-14, IFNa-17, IFNa-7, IFNa- 6、IFNa-4b、IFNa-16，分别）
