产品详情
  • 产品名称:Monoclonal anti Calbindin D-28k 200 µl

  • 产品型号:300
  • 产品厂商:Swant
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
瑞士Swant​公司,瑞士一家生物科技公司,产品包括用于钙结合蛋白(D-28k),钙调蛋白,离子通道蛋白,神经递质的抗体和抗原,用于神经元或神经胶质细胞及癌细胞的染色产品和用于免疫组合的单抗和多抗。多年来专注于钙结合蛋白产品的研究,其产品虽种类虽然不多,但是却都经典热销,并且经常出现在高分的文章中。为国内外众多相关领域科学家所热衷。
详情介绍:

产品介绍

名称

 Monoclonal anti Calbindin D-28k 

货号

 300

规格

 200ul / 500ul  / 1ml

 克隆性

 monoclonal

共轭

 nonconjugated

反应性

 thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque

应用

 western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

宿主动物

 Mouse monoclonal, IgG1

抗体形式

 Conc. supernat. lyophil.

品形式

 Lyophilized concentrated supernatant (no preservatives). 

复溶

 with 200 µl / 500 µl or 1 ml bidistilled water

产品详情

产品描述

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

稀释

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

We recommend that the optimal dilutions be determined by titration experiments.

贮存

 For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

参考资料

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

 
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