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产品介绍 |
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| 名称 |
Monoclonal anti Calbindin D-28k |
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| 货号 |
300 |
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| 规格 |
200ul / 500ul / 1ml |
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| 克隆性 |
monoclonal |
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共轭 |
nonconjugated |
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| 反应性 |
thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque |
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| 应用 |
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section |
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| 宿主动物 |
Mouse monoclonal, IgG1 |
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| 抗体形式 |
Conc. supernat. lyophil. |
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| 产品形式 |
Lyophilized concentrated supernatant (no preservatives). |
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| 复溶 |
with 200 µl / 500 µl or 1 ml bidistilled water |
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产品详情 |
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| 产品描述 |
Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C |
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| 稀释 |
Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments. |
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| 贮存 |
For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. |
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| 参考资料 |
1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493 |
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产品详情
简单介绍：
瑞士Swant公司，瑞士一家生物科技公司，产品包括用于钙结合蛋白（D-28k），钙调蛋白，离子通道蛋白，神经递质的抗体和抗原，用于神经元或神经胶质细胞及癌细胞的染色产品和用于免疫组合的单抗和多抗。多年来专注于钙结合蛋白产品的研究，其产品虽种类虽然不多，但是却都经典热销，并且经常出现在高分的文章中。为国内外众多相关领域科学家所热衷。
详情介绍：
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