产品详情
  • 产品名称:Mouse anti-calretinin (monoclonal) 1 ml

  • 产品型号:6B3
  • 产品厂商:Swant
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
品牌:Swant 货号:6B3 品名:Mouse anti-calretinin (monoclonal) 规格:1ml
详情介绍:

产品介绍

名称

Mouse anti-calretinin (monoclonal)

货号

6B3

规格

1ml

 克隆性

monoclonal

共轭

nonconjugated 

反应性

Erpetoichthys calabaricusslender lungfishPolypterus senegalusMongolian jirdhumanmouseratzebrafish Caenorhabditis elegansrhesus macaque

应用

western blotimmunohistochemistryimmunocytochemistryimmunoprecipitationimmunohistochemistry - paraffin sectionimmunohistochemistry - frozen sectionimmunohistochemistry - free floating section

宿主动物

Mouse monoclonal

抗体形式

Conc. supernat. lyophil.

产品形式

Lyophilized concentrated supernatant (no preservatives). 

复溶

each vial with 200 µl of bidistilled water

产品详情

描述

The antibody against calretinin was produced in mice by immunization with recombinant human calretinin-22k (1). Calretinin 22k is an alternative splice product of the calretinin gene and identical with calretinin up to Arg178. After fusion, hybridoma cells were screened with human recombinant calretinin as target and the clone 6B3 was selected. The antibody 6B3 recognizes an epitope within the first 4 EF-hands domains common to both calretinin and calretinin-22k (1). It was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde (or 10% buffered formalin) fixed brains b) by immunoenzymatic labelling of immunoblots c) by immunohistochemistry on tissue of calretinin knock-out mice.

稀释

Immunohistochemistry: 1:2'000 - 1:5'000. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate or paraffin-sections. 

Immunoblots: 1:1'000 - 1:2'000. 

For immunohistochemistry and immunoblots the titer was determined by using the avidinbiotin method. We recommend that the optimal dilutions be determined by titration experiments.

贮存

After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

参考资料

1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibodies recognizes epitopes of calretinin. Cell Calcium 31: 13-25. 

2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol. 105:1343-1353. 

3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-147. 

4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046. 

5. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.

 
标题:
内容:
联系人:
联系电话:
Email:
公司名称:
联系地址:
 
 
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
相关文章

沪公网安备 31011202007343号