Streptavidin-HRP HRP标记的链霉亲和素

Formulation	Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Reconstitution	Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml.
Storage	The reagent may be stored at 4°C for short periods of one month or less. For longer periods,aliquot and store at -20°C . Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points for this product:
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:20,000
ELISA: 1:50,000-1:200,000
Others: User-optimized

Target Background

Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin.
Streptavidin-HRP (M00091) is coupled steptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.

HRP标记的链霉亲和素

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报