Product Details
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Product Name
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Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [RM120]
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Product Description
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Biotinylated Rabbit monoclonal to Human IgG4
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Catalog No.
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31-1022-02
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Clone Name
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RM120
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Product Data Sheet
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Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
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Specificity
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RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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Immunogen
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Peptide corresponding to the hinge region of Human IgG4
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Western Blot
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Species Reactivity
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Human
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Conjugate
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Biotin
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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50 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
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Keywords
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Product Images
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Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
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Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
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Western Blot of human, mouse, rat, and goat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.
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A titer ELISA using RM120. The plate was coated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG4 antibody RM120.
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