Product Details
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Product Name
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Anti-Mouse IgG2a Rabbit Monoclonal Antibody [RM219]
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Product Description
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Rabbit monoclonal to Mouse IgG2a
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Catalog No.
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31-1098-00
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Clone Name
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RM219
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Product Data Sheet
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Anti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219
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Specificity
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RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
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Immunogen
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Mouse IgG2a
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Application
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Western Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Mouse
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.5 ug/mL-2 ug/mL.
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Keywords
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Product Images
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ELISA of mouse immunoglobulins shows RM219
reacts to the Fc region of mouse IgG2a; no cross
reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
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A titer ELISA of mouse IgG2a. The plate was
coated with different amounts of mouse IgG2a. A
serial dilution of RM219 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
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Western blot of nonreduced(-) and reduced(+)
mouse IgG2a, using 0.5ug/mL of RevMAb clone
RM219. This antibody reacts to nonreduced
IgG2a (~150 kDa).
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