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Product Details
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Product Name
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Anti-His-Tag Chimeric Human Monoclonal Antibody, [RMH01]
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Product Description
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Chimeric Human monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
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Catalog No.
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54-1161-00
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Clone Name
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RMH01
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Product Data Sheet
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Anti-His-Tag Chimeric Human Monoclonal Antibody Data Sheet RMH01
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Specificity
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RMH01 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
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Immunogen
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Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
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Application
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Western Blot
ELISA (Capturer or Detector)
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Species Reactivity
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All
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: Capturer: 0. 1 ug/mL – 2 ug/mL;
Detector: 0.01 ug/mL – 0.5 ug/mL;
Western Blot: 0.1 ug/mL – 1 ug/mL.
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Keywords
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Product Images
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Sandwich ELISA performed on different amounts of
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A titer ELISA performed on purified HisTag-PCSK9
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Western blot of 293T cells transfected (+) or untransfected (-)
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