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Product Details
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Product Name
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Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM172]
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Product Description
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Rabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation)
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Catalog No.
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31-1056-00
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Clone Name
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RM172
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Product Data Sheet
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Anti-Acetyl-Histone H3 Lys27 Rabbit Monoclonal Antibody Data Sheet RM172
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Specificity
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RM172 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3.
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Immunogen
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An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).
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Application
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Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Chromatin IP (ChIP)
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Species Reactivity
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Human
Vertebrates
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Keywords
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References for Acetyl-Histone H3 (Lys27) antibody [RM172]
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Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794)
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Product Images
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RM172 specifically reacts to Histone H3
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Western Blot of acid extracts from HeLa cells
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Immunocytochemistry of HeLa cells treated with
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