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Product Details
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Product Name
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Anti-Histone H3 K27M Rabbit Monoclonal Antibody [RM192]
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Product Description
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Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant
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Catalog No.
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31-1175-00
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Clone Name
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RM192
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Product Data Sheet
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Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192
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Specificity
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RM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3.
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Immunogen
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A peptide corresponding to Histone H3 K27M mutant
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Application
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Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
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Species Reactivity
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All
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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50 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.01 ug/mL – 1 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.005 – 0.1 ug/mL;
Immunocytochemistry (ICC/IF): 0.005 – 0.1 ug/mL.
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References for Histone H3 K27M Mutant antibody [RM192]
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Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor
Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
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Product Images
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Immunostaining of brain tumor tissue sections
Image courtesy of Sebastian Brandner MD, Division
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Immunostaining of brain tumor tissue sections
Image courtesy of Sebastian Brandner MD, Division
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Western Blot analysis of cell lysates prepared from
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Immunohistochemical staining of formalin fixed
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