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Product Details
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Product Name
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Human IgG4 Matched Antibody Pair
Rabbit Monoclonal
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Product Description
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The Human IgG4 Matched Antibody Pairs is specific to Human IgG4 and has been demonstrated through ELISA to not recognize (cross) IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), nor does the matched pair recognize mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data.
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Catalog No.
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31-1022-MK
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Clone Name
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RM120 Anti-Human IgG4 Rabbit Monoclonal Antibody
RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
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Product Data Sheet
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Human IgG4 Matched Antibody Pair
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Specificity
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The antibody pair detects only human IgG4. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
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Application
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Sandwich ELISA, or other Sandwich Assays
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Species Reactivity
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Human
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Conjugate
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RM129: Biotin
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Components
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1) Capture Antibody: Anti-Human IgG4 Rabbit Monoclonal antibody, clone RM120, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Keywords
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Usage
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ELISA: Capture Antibody 50ng/well – 200ng/well;
Detection Antibody 0.1ug/mL – 0.5ug/mL
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Product Images
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Sandwich ELISA, using RevMAb human IgG4
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Detection of human IgG4 in monkey serum.
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Detection of human IgG4 in mouse or rat serum.
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Detection of human IgG4 in mouse or rat serum.
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