Product Details
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Product Name
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Anti-Phospho-Histone H3 (Ser10) Rabbit Monoclonal Antibody [RM163]
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Product Description
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Rabbit monoclonal to Phospho-Histone H3 (Ser10), Histone H3 (S10 phosphorylation)
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Catalog No.
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31-1070-00
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Clone Name
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RM163
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Product Data Sheet
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Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody Data Sheet RM163
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Specificity
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RM163 reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones.
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Immunogen
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A phospho-peptide corresponding to Phospho-Histone H3 (Ser10).
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Application
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Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
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Species Reactivity
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Human
Vertebrates
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Conjugate
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None
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Intended Use
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Research Use Only
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.
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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0.5 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.
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Keywords
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.
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Product Images
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Western Blot of acid extracts of HeLa cells treated
or non-treated with Nocodazole. Using RM163 at
0.5 ug/mL, showed a band of Histone H3 phospho-
rylated at serine 10 in HeLa cells.
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Immunocytochemistry of HeLa cells using Phospho-Histone H3
(Ser10) Rabbit mAb RM163 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).
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