Product Details
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Product Name
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Anti-Human IgA2 Rabbit Monoclonal Antibody [RM125]
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Product Description
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Rabbit monoclonal to Human IgA2
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Catalog No.
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31-1027-00
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Clone Name
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RM125
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Product Data Sheet
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Anti-Human IgA2 Rabbit Monoclonal Antibody Data Sheet RM125
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Specificity
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RM125 reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
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Immunogen
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Human IgA2
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Human
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Conjugate
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None
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Intended Use
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Research Use Only
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.
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
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Keywords
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Product Images
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Immunohistochemistry of Human Tonsil Tissue using
Anti-Human IgA2 antibody RM125.
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Sandwich ELISA using RM125 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA using RM125 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
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ELISA of human immunoglobulins shows RM125
reacts only to Human IgA2. No cross reactivity
with Human IgA1, IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM125 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
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A titer ELISA using RM125. The plate was coated
with different amounts of human IgA2. A serial dilution
of RM125 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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