Product Details
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Product Name
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Anti-Human IgD Rabbit Monoclonal Antibody [RM123]
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Product Description
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Rabbit monoclonal to Human IgD
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Catalog No.
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31-1025-00
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Clone Name
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RM123
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Product Data Sheet
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Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123
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Specificity
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RM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA.
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Immunogen
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Human IgD
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Human
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Conjugate
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None
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Intended Use
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Research Use Only
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.
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 25ng/well – 200ng/well (for Capture);
0.01ug/mL – 0.1ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Keywords
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.
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Product Images
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Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgD antibody RM123.
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Sandwich ELISA using RM123 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
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ELISA of human immunoglobulins shows RM123
reacts to IgD from human plasma and IgDκ from
human myeloma. No cross reactivity with human
IgG, IgM, IgA, or IgE. The plate was coated with
50 ng/well of different immunoglobulins. 200 ng/mL,
50ng/mL, or 10 ng/mL of RM123 was used as the
primary antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
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A titer ELISA using RM123. The plate was coated
with different amounts of human IgD (from plasma).
A serial dilution of RM123 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
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