Product Details
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Product Name
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Anti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [RM171]
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Product Description
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Rabbit monoclonal to Dimethylated Histone H3 Lysine 23, H3K23me2, Histone H3 (dimethyl K23)
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Catalog No.
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31-1077-00
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Clone Name
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RM171
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Product Data Sheet
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Anti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody Data Sheet RM171
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Specificity
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RM171 reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23, monomethylated Lysine 23 (K23me1), or other methylations in histone H3.
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Immunogen
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A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys23).
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Application
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Western Blot (WB)
ELISA
Multiplex
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Species Reactivity
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Human
Vertebrates
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.1 ug/mL - 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Keywords
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Product Images
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A Peptide dotblot shows RM171 only reacts to Histone.
H3 dimethyl-Lysine 23 (K23me2). No cross reactivity.
with nonmodified Lysine 23 (K23Ctrl), monomethylated.
Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
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RM171 specifically reacts to Histone H3 dimethylated
at Lysine 23 (K23me2). No cross reactivity with other
methylated lysines in Histone H3.
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Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM171
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 23 (K23me2) in HeLa cells.
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