Product Details
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Product Name
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Anti-Histone H3 pan Rabbit Monoclonal Antibody [RM188]
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Product Description
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Rabbit monoclonal to Histone H3 pan
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Catalog No.
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31-1057-00
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Clone Name
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RM188
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Product Data Sheet
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Anti-Histone H3 pan Rabbit Monoclonal Antibody Data Sheet RM188
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Specificity
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RM188 reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins
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Immunogen
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A peptide corresponding to the C-terminus of human Histone H3.
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Application
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Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
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Species Reactivity
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Human
Vertebrates
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.01 ug/mL – 0.25 ug/mL;
ICC: 1 ug/mL - 5 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Keywords
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.
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Product Images
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RM188 reacts to both Histone H3.1 and Histone H3.3.
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Western Blot of recombinant Histone H3.3 and
Histone H3.1 proteins, A375, HEK293, HeLa
and SK-MEL-2 whole cell lysates, using RM188
at 0.025 ug/mL, showed a band of recombinant
H3.1 and H3.3 proteins, and total histone
H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
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Immunocytochemistry of HeLa cells, using Histone
H3 Rabbit mAb RM188 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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Sandwich ELISA against acetylated histone H3 at Lys 9
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the
capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.
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Sandwich ELISA against acetylated histone H3
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 ug/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 ug/mL) as the
detection antibody.
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