Product Details
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Product Name
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Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [RM195]
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Product Description
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Rabbit monoclonal to Monomethylated Histone H4 Arginine 3, H4R3me1, Histone H4 (monomethyl R3)
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Catalog No.
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31-1080-00
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Clone Name
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RM195
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Product Data Sheet
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Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195
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Specificity
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RM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
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Immunogen
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A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).
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Application
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Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
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Species Reactivity
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Human
Vertebrates
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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100 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
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Keywords
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Product Images
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RM195 specifically reacts to Histone H4 mono-
methylated at Arginine 3 (R3me1). No cross reactivity
with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated
Arg 3 (R3me2a) or symmetric dimethylated Arg 3
(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
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Western Blot of (1) acid extracts of HeLa cells; (2)
recombinant Histone H4. Using RM195 at 0.5 ug/mL,
showed a band of Histone monomethylated at Arginine
3 in HeLa cells.
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Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Monomethyl-Histone H4
(Arg3) Rabbit Monoclonal Antibody (clone RM195) (red).
Actin filaments have been labeled with fluorescein
phalloidin (green).
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