Product Details
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Product Name
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Anti-Mouse IgG2b Rabbit Monoclonal Antibody [RM108]
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Product Description
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Rabbit monoclonal to Mouse IgG2b
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Catalog No.
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31-1004-00
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Clone Name
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RM108
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Product Data Sheet
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Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108
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Specificity
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RM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
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Immunogen
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Mouse IgG2b
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Application
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Western Blot (WB) (stronger on nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Mouse
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
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Keywords
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Product Images
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Western blot of nonreduced(-) and reduced(+)
mouse IgG2b (20ng/lane), using 0.2ug/mL of
RevMAb clone RM108. This antibody reacts to
nonreduced IgG2b (~150 kDa) much stronger
than the reduced γ2b form (~50 kDa).
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ELISA of mouse immunoglobulins shows RM108
reacts to the Fc region of mouse IgG2b; no cross
reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was
used as the primary antibody. An alkaline phosph-
atase conjugated anti-rabbit IgG as the secondary
antibody.
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A titer ELISA of mouse IgG2b. The plate was
coated with different amounts of mouse IgG2b.
A serial dilution of RM108 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
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