Product Details
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Product Name
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Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody [RM110]
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Product Description
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Rabbit monoclonal to Mouse Ig Lambda Light Chain
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Catalog No.
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31-1006-00
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Clone Name
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RM110
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Product Data Sheet
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Anti-Mouse Ig Lambda Rabbit Monoclonal Antibody Data Sheet RM110
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Specificity
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RM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM110 has been engineered to eliminate Fc receptor binding.
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Immunogen
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Mouse IgM lambda
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Application
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Western Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Mouse
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 0.005 ug/mL-0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.1ug/mL-0.5 ug/mL
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Keywords
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Product Images
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Western blot of nonreduced(-) and reduced(+)
mouse IgG1κ and IgG1λ (20ng/lane), using
0.2ug/mL of RevMAb clone RM110. This antibody
reacts to nonreduced IgG1λ.
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ELISA of mouse immunoglobulins shows RM110
reacts to the lambda light chain of mouse immuno-
globulins. No cross reactivity with the kappa light
chain, human IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immuno-
globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of
RM110 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.
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A titer ELISA of mouse IgG1λ. The plate was coated
with different amounts of mouse IgG1λ. A serial
dilution of RM110 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.
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