Product Details
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Product Name
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Anti-Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
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Product Description
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Rabbit monoclonal to Phosphotyrosine
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Catalog No.
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32-1011-00
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Clone Name
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RM111
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Product Data Sheet
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Anti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111
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Specificity
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RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
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Immunogen
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Phosphotyrosine-BSA conjugate
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Application
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Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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All Species
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Conjugate
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None
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Intended Use
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Research Use Only
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.
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot (WB): 1:1000 -1:5000 dilution;
Immunoprecipitation (IP): 1:200-1:1000 dilution;
Chromatin IP (ChIP): 1:200-1:1000 dilution;
Immunocytochemistry (ICC): 1:200-1:500 dilution;
Immunohistochemistry (IHC): 1:200-1:500 dilution.
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Keywords
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References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
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Sun et al., Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity, Neuron (2016), http://dx.doi.org/
10.1016/j.neuron.2016.08.033. IHC; Mouse Read more (PubMed: 27641496)
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Product Images
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Immunocytochemistry of serum-starved A431
cells nontreated or treated with EGF, using
RevMAb Clone RM111 at 1/500 dilution (foll-
owed by a PE conjugated secondary antibody,
red) and DAPI (blue).
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Western blot of serum-starved A431 cells non-
treated or treated with EGF, using RevMAb
Clone RM111 at 1/5000 dilution. The blot was
exposed to the film at different time points.
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Immunoprecipitation of EGF-treated A431 cells
by RevMAb Cone RM111 at 1/1000 dilution, was
blotted with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control; (2) IP by rabbit IgG control;
(3) IP by Clone RM111.
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Luminex analysis of Clone RM111’s reaction
against phosphorylated or nonphosphorylated
Serine, Threonine, and Tyrosine. RevMAb Clone
RM111 reacts only to phosphorylated Tyrosine.
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