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- 产品名称:Calbindin D-9k 60 µg
- 产品型号:rCB9
- 产品展商:Swant
- 产品文档:无相关文档
- 发布时间:2024-05-07
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简单介绍
品牌:Swant
货号:rCB9
品名:Calbindin D-9k
规格:60 µg
产品描述
产品介绍
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品名
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Rat recombinant calbindin D-9k (recombinant) produced in Escherichia Coli
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货号
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rCB9
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规格
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10 µg、60 µg
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纯化
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Ni-chelate chrom
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宿主动物
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Recombinant rat
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抗体形式
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lyophil
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重组
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10 µg protein reconstituted with 25 µl PBS (0.4 µg/µl)
60 µg protein reconstituted with 150 µl PBS
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产品描述
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E. coli containing a His6-fusion protein were disrupted in a buffer containing 6M GuHCl/0.1 M NaH2PO4. After a low speed centrifugation to remove cell debris, the supernatant was applied to the charged NTA resin column, equilibrated in disruption buffer, before the column being washed with the same buffer. Afterwards, the column was washed and the HIS6- fusion proteins eluted by decreasing the pH of the eluent (8 M Urea /0.1 M Na2HPO4) in a stepwise manner (pH 6.3 / 5.9 / 4 / 2). The protein was dialysed against phosphate buffered saline, 0.1 M, pH 7.3 (PBS)
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储存
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Freeze in small aliquots (e.g. 1 or 10 µg) and keep at -80°C. Avoid repeated freezing and thawing
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