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- 产品名称:Rabbit anti Calbindin D-28k 500 µl
- 产品型号:CB38
- 产品展商:Swant
- 产品文档:无相关文档
- 发布时间:2024-05-07
- 在线询价
简单介绍
品牌:Swant
货号:CB38
品名:Rabbit anti Calbindin D-28k
规格:500UL
产品描述
产品介绍
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品名
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Rabbit anti-Calbindin D-28k
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货号
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CB-38a (new batch)
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规格
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500 µl
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克隆性
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polyclonal
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共轭
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nonconjugated
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反应性
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human, mouse, rat, pigs
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应用
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western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
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宿主动物
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Rabbit
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抗体形式
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Antiserum lyophil.
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产品形式
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Lyophilized antiserum (no preservatives).
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复溶
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with 200 µl bidistilled water
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产品描述
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This antiserum was produced against recombinant rat calbindin D-28k (CB). It crossreacts with calbindin D-28k from many other species, including human, monkey, rat, mouse chicken and fish (Fig. 1). In immunoblots it recognizes a single band of approximately 27 -28 kDa. In samples with very high (10-fold higher than CB) concentrations of the related protein calretinin, a larger band (29 - 30 kDa) may appear in Western blots. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium-binding protein could be also revealed. In case of doubt, we suggest to switch to Swant’s monoclonal antibody 300 directed against calbindin D-28k, which does not recognize calretinin.
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稀释
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Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method.
Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments.
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储存
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Make small portions (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
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参考文献
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1. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
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