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- 产品名称：Affinity purified mouse anti-calretinin (monoclonal)
- 产品型号：6B3PUR
- 产品展商：Swant
- 产品文档：无相关文档
- 发布时间：2024-05-07
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简单介绍
品牌：Swant
货号：6B3PUR
品名：Affinity purified mouse anti-calretinin (monoclonal)
规格：100ul
产品描述
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产品介绍
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品名
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Affinity purified mouse anti-calretinin (monoclonal)
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货号
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6B3PUR
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规格
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100ul
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形式
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Purified antibody in PBS (lyophilized).
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复溶
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with 100 µl bi-distilled water
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描述
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The antibody 6B3pur was purified with Protein-G from in vitro produced monoclonal
antibody 6B3 directed against calretinin (see product description 6B3 for further details
concerning immunoblot and immunohistochemistry). The antibody concentration is of
0.29 mg/ml in 0.1M phosphate buffered saline, pH 7.3.
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稀释
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Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.029 g/ml) for
immunostaining with the avidin-biotin method on cryostat or paraffin-sections of
paraformaldehyde (4%) or formalin-fixed tissue.
We recommend that the optimal dilutions be determined by titration experiments
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储存
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After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least -
20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing
and thawing.
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参考文献
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1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibois recognizes epitopes of calretinin. Cell Calcium 31: 13-25.
2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol.
105:1343-1353.
3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the
epithelial type. Pathol. Res. Pract. 192:137-147.
4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol.
20:1037-1046.
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