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- 产品名称:Rabbit anti-calretinin 500 µl
- 产品型号:7697
- 产品展商:Swant
- 产品文档:无相关文档
- 发布时间:2024-05-07
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简单介绍
品牌:Swant
货号:7697
品名:Rabbit anti-calretinin
规格:500 µl
产品描述
产品介绍
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品名
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Rabbit anti-calretinin
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货号
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7697
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规格
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500 µl
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克隆性
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polyclonal
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共轭
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nonconjugated
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反应性
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human, mouse, leopard danio
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应用
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immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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宿主动物
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Rabbit
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抗体形式
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Antiserum lyophil.
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产品形式
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Lyophilized whole serum (no preservatives).
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复溶
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with 200 µl of bidistilled water
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产品描述
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The antiserum against calretinin is produced in rabbits by immunization with recombinant human calretinin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoenzymatic labelling of immunoblots。
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稀释
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Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections.
Immunoblots: 1:1'000 - 1:2'000.
For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.
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储存
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After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
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参考文献
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1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.
2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.
3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137- 147.
4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
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