名称：重组小鼠IL-22
Synonyms：IL-TIF, IL-TIF alpha, IL-22a
Accession：Q9JJY9
GeneID：50929
Source：Escherichia coli.
Molecular Weight：Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Quantity：2µg/10µg/1000µg
AA Sequence：LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK KLGESGEIKA IGELDLLFMS LRNACV
Purity：> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity：Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.
Endotoxin：Less than 1 EU/μg of rMuIL-22 as determined by LAL method.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference：1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.
2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.
3. Honda K. 2012. Immunity, 37: 952-4.
4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.
Background：IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells. IL-22 likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant mouse interleukin-22 contains 147 amino acids and it has approximately 81 % and 92 % amino acid sequence identity with human and rat IL-22.
SDS-PAGE：
