名称：重组小鼠IL-36RA
Synonyms：IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1
Accession：Q9QYY1
GeneID：54450
Source：Escherichia coli.
Molecular Weight：Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
Quantity：2µg/10µg/1000µg
AA Sequence：VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCD
Purity：> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity：Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin：Less than 1 EU/µg of rMuIL-36RA as determined by LAL method.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference：1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528.
2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44.
3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308.
4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6.
5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602.
Background：Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant murine interleukin-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with human IL-36RA.