名称：重组牛GCP-2/CXCL6
Accession：P80221
GeneID：281735
Unigene：Bt.7165.
Source：Escherichia coli.
Molecular Weight：Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
AA Sequence：GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN
Purity：> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity：Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/ml.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.
Endotoxin：Less than 0.1 EU/µg of rBoGCP-2/CXCL6 as determined by LAL method.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background：GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.