名称:重组人Tβ-4
Synonyms:Fx
Accession:P62328
GeneID:7114
Source:Escherichia coli.
Molecular Weight:Approximately 4.9 kDa, a single non-glycosylated polypeptide chain containing 43 amino acids.
Quantity:20µg/100µg/1000µg
AA Sequence:SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES
Purity:> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 μg/ml.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4.
Endotoxin:Less than 1 EU/µg of rHuTβ4 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference:1. Moon EY, Song JH, Yang KH. 2007. Oncol Res, 16: 507-16.
2. Oh SY, Song JH, Gil JE, et al. 2006. Exp Cell Res, 312: 1651-7.
3. Oh JM, Ryoo IJ, Yang Y, et al. 2008. Cancer Lett, 264: 29-35.
Background:Thymosin Beta 4 is a naturally occurring peptide encoded by the TMSB4X gene located on Chr. X in humans. It is found in high concentrations in blood platelets, wound fluid and other tissues in the body. Tβ-4 is a major actin regulating peptide and the primary function is to stimulate the productions of T cells, which plays important part of the immune system. The thymosin beta-4 peptide, if used after a heart attack, might reactivate cardiac progenitor cells to repair damaged heart tissue.
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