图1.the MasterPure™ Complete Kit流程概述
图2. 使用MasterPure™完整DNA和RNA纯化试剂盒提纯的模板与传统离心柱型试剂盒提纯的模板,比较PCR敏感性。从一系列稀释的大肠杆菌细胞(200 to 2 x 107 )纯化核酸后进行PCR反应泳道1-6。泳道M, DNA marker; 泳道 7, 阴性对照l.
图3(点击看大图)。使用MasterPure™完整DNA和RNA纯化试剂盒从不同的细胞来源中纯化总核酸(TNA)。 M 为kb ladder。
Targets
Samples Extracted
Human & mammalian RNA & genomic DNA HIV HCV RSV HPV Yeast Enterovirus B. pertussis E. coli M. tuberculosis Streptococcus mutans Soy (tofu) Maize Insect tissues
Plasma Whole blood Guthrie cards Buccal cells Liver Mouse tail Kidney Saliva Urine Sputum Tissue-culture cell lines Cervical cells Paraffin-embedded tissues
表1. 可使用 MasterPure™ DNA 和RNA 纯化试剂盒的目标和样本举例。应用比较简单的盐沉淀步骤在1小时内从任何来源的样品中分离TNA,DNA或RNA。
Sample
Sample Size
TNA µg
DNA µg
RNA µg
HeLa/HL60 cells
1 X 106 cells
10-30
3-12
7-15
Tissues
Liver
5 mg
33-42
5-10
13-25
Brain
9-13
6-9
4-11
Heart
6-10
4-7
4-5
Kidney
10-17
3-8
14-17
Thymus
15-30
6-12
9-18
Blood
200 µl
3-10
3-9
Buffy coat
300 µl
40-55
3-6
Mouse tail
0.5 cm
25-30
9-11
E. coli
3.5 x 106 cells
2.5-2.8
1.3-1.6
1.6-1.8
S. mutans
1.5 ml
0.9
Yeast*
(S. cerevisiae)
2.2 x 106 cells
11-18
1.1 x 107 cells
70-78
*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.
Table 2. 举例说明使用MasterPure™完整DNA和RNA纯化试剂盒获得的核酸产量
图4. 纯化DNA,TNA,RNA的一致性。使用MasterPure™完整DNA和RNA纯化试剂盒从五种不同的冷冻的牛肝中分离DNA(泳道1-5),TNA(泳道6-10),及总细胞RNA(泳道11-15),泳道M, Kilobase ladder.
MasterPure™ Individually Packaged Bulk Reagents (10X Package Size)
The following reagents are designed for use with the MasterPure™ kit protocols only.