产品简介:北京中北林格供应lucigen 30810-2 SelecTEV™ Protease 产品说明书,中北林格是lucigen总代理,提供lucigen产品货号报价查询。代理的其他品牌包括Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>
产品名称:SelecTEV™ Protease</p>
货号:lucigen 30810-2</p>
规格:5,000 U</p>
存储温度:-20º C</p>
品牌:lucigen</p>
供应商:中北林格</p>
产地:us</p>
发货地:北京</p>
SelecTEV™ Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease. SelecTEV™ Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. This seven-amino-acid sequence is rarely found in proteins, making SelecTEV™ Protease an ideal choice for tag removal of fusion proteins. The optimal temperature for cleavage is 30°C, however the enzyme is active over a wide temperature range (4 – 30°C) and pH (6.0 – 8.5) to accommodate your specific protein. Following digestion, SelecTEV™ Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease. SelecTEV™ Protease is purified from E. coli by affinity chromatography using the polyhistidine tag and is 90% pure when visualized on an SDS-PAGE gel.
SelecTEV Protease can be used with the Expresso Solubility and Expression Screening System vectors, which contain a TEV cleavage recognition site.
SelecTEV™蛋白酶是一种27 kD改良形式的**蚀纹病毒(TEV)蛋白酶,经过设计,比天然蛋白酶更稳定,更有活性和特异性。SelecTEV™蛋白酶是半胱氨酸蛋白酶,其识别TEV识别序列Glu-Asn-Leu-Tyr-Phe-Gln-Gly并在Gln和Gly之间切割。这种七氨基酸序列很少见于蛋白质,使SelecTEV™蛋白酶成为融合蛋白标签去除的理想选择。zui佳裂解温度为30°C,但酶在较宽的温度范围(4 - 30°C)和pH(6.0 - 8.5)范围内具有活性,可以适应您的特定蛋白质。消化后,使用蛋白酶N-末端的多组氨酸标签通过亲和层析容易地从切割反应中除去SelecTEV TM蛋白酶。SelecTEV™蛋白酶经过纯化 使用多组氨酸标签通过亲和层析进行大肠杆菌,并且在SDS-PAGE凝胶上观察时纯度为90%。
SelecTEV蛋白酶可与Expresso溶解度和表达筛选系统载体一起使用,其含有TEV切割识别位点。
Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3)
30810-1: SelecTEV Protease (1 tube), 100 mM DTT (1 tube), 20X SelecTEV Buffer (1 tube)
30810-2: SelecTEV Protease (5 tubes), 100 mM DTT (5 tubes), 20X SelecTEV Buffer (5 tubes)
30810-1: SelecTEV蛋白酶(1管),100 mM DTT(1管),20X SelecTEV缓冲液(1管)
30810-2: SelecTEV蛋白酶(5管),100 mM DTT(5管),20X SelecTEV缓冲液(5管)