ScriptCap™ m7G Capping
System C-SCCE0625 25 reactions
产品简介：北京中北林格供应ScriptCap™ m7G Capping System
C-SCCE0625 25 reactions，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch
Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
ScriptCap™ m7G Capping System C-SCCE0625 25 reactions 品牌介绍：
CELLSCRIPT专注于再生医学，细胞再sheng学以及细胞疗法。细胞、组织或器官的移植治liao及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。
The mission of CELLSCRIPT™ is to provide the best products and technologies for making and using RNA for translation in cells for clinical research and therapeutics.
产品介绍：For post-transcriptional capping of in vitro transcribed RNA with
ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built
with efficiencies approaching 100%. This cannot be accomplished with
co-transcriptional capping methods. Use in conjunction with ScriptCap
2'-O-Methyltransferase to produce
Cap 1 caps, that
which is found on most eukaryotic mRNAs.</p>
产品规格
名称：ScriptCap™ m7G Capping System
C-SCCE0625 25 reactions</p>
分类：Post-Transcriptional Capping</p>
货号：C-SCCE0625</p>
规格：25 reactions</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
运输方式：送货上门/顺丰</p>
是否现货：期货</p>
ScriptCap™ m7G Capping System C-SCCE0625 25 reactions 产品详情
ScriptCap™ m7G Capping
System catalyzes in vitro addition of a cap nucleotide to the 5' terminus of
primary RNA that has a 5'-triphosphate group, such as RNA obtained from an in
vitro transcription reaction. A "cap nucleotide" or “cap” is a guanine nucleoside that is joined
via its 5' carbon to a triphosphate group that is, in turn, joined to the 5'
carbon of the most 5' nucleotide of the primary mRNA transcript, and in most
eukaryotes, the nitrogen at the 7 position of guanine in the cap nucleotide is
methylated. Such a capped transcript can be represented as:
m7G(5')ppp(5')N1(pN)x-OH(3') where m7G represents the 7-methylguanosine cap
nucleoside, ppp represents the triphosphate bridge between the 5' carbons of
the cap nucleoside and the first nucleotide of the primary RNA transcript, and
N1(pN)x-OH(3') represents the primary RNA transcript, of which N1 is the most
5' nucleotide. The ScriptCap m7G Capping System sequentially catalyzes three
different enzymatic reactions:
(1) RNA
triphosphatase cleaves the 5' triphosphate of RNA to a
diphosphate.pppN1(p)Nx-OH(3') ppN1(pN)x-OH(3') + Pi
(2) RNA guanyltransferase
joins GTP to the 5' diphosphate of the most 5' nucleotide (N1) of the
RNA.ppN1(pN)x-OH(3') + GTP G(5')ppp(5')N1(pN)x-OH(3') + PPi
(3)
Guanine-7-methyltransferase, using S-adenosyl-methionine (AdoMet) as a
co-factor, catalyzesmethylation of the 7-nitrogen of guanine in the cap
nucleotide.
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ScriptCap™ m7G Capping System C-SCCE0625 25 reactions 关键词
ScriptCap™ m7G Capping System</p>
cellscript ScriptCap™ m7G Capping System</p>
cellscript总代理 ScriptCap™ m7G Capping System</p>
cellscript总代理中北林格 ScriptCap™ m7G Capping System</p>
cellscript C-SCCE0625 25 reactions</p>
cellscript总代理 C-SCCE0625 25 reactions</p>
cellscript总代理中北林格 C-SCCE0625 25 reactions</p>
cellscript Post-Transcriptional Capping C-SCCE0625</p>
cellscript总代理 Post-Transcriptional Capping C-SCCE0625</p>
cellscript总代理中北林格 Post-Transcriptional Capping C-SCCE0625</p>
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