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INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510
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  • 产品名称:INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510
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简单介绍
北京中北林格供应INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510,中北林格是cellscript总代理,提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理,epicentre总代理,lucigen总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510
产品描述

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510</p>

产品简介:北京中北林格供应INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510,中北林格是cellscript总代理,提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理,epicentre总代理,lucigen总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>


名称:INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510</p>

分类:Modified NTP In Vitro Transcription</p>

货号:C-ICTMY110510</p>

规格:10 reactions</p>


产品详情:For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.</p>


供应商:中北林格</p>

产地:us</p>

发货地:北京</p>

运输方式:送货上门/顺丰</p>

是否现货:期货</p>


INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510 产品详情

INCOGNITO™T7 5mC-Ψ-RNA转录试剂盒  PDF

T7 RNA聚合酶生产含有5-甲基胞嘧啶 - 假尿苷的体外转录RNA5mC-Ψ-mRNAs在哺乳动物细胞中诱导较低的先天免yi反应。反应在2小时内从1μgDNA产生100μgRNA

The INCOGNITO™ T7 5mC- & -RNA Transcription Kit* is optimized for high-yield synthesis of 5-methylcytosine- pseudouridine-containing RNA (5mC -RNA) by in vitro transcription (IVT) of a DNA template that has a properly-oriented phage T7 RNA polymerase promoter using T7-Scribe™ RNA polymerase, the canonical nucleotides ATP & GTP, and the modified nucleotides 5-methylcytosine-5'-triphosphate (5mCTP) & pseudouridine-5' triphosphate ( TP). Although yield varies with the DNA template and other factors, a standard 20 μl IVT reaction using 1 μg of a control template DNA yields approximately 100 μg of 5mC -RNA in 2 hours at 37oC. The 5mC -RNA can be enzymatically 5'-capped and 3'-poly(A)-tailed using CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap™ 2'-O-Methyltransferase and A-Plus™ Poly(A) Polymerase to generate 5mC -mRNA for expression in cells. It has been shown that 5mC -mRNAs are translated into protein at higher levels and induce lower innate immune responses in human and other mammalian cells that express various RNA sensors compared to corresponding unmodified mRNAs.

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit C-ICTMY110510 其他关键词:</p>

cellscript INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit</p>

cellscript总代理 INCOGNITO T7 5mC- & Ψ-RNA Transcription Kit</p>

cellscript总代理中北林格 INCOGNITO T7 5mC- & Ψ-RNA Transcription Kit</p>

cellscript C-ICTMY110510 10 reactions</p>

cellscript总代理 C-ICTMY110510 10 reactions</p>

cellscript总代理中北林格 C-ICTMY110510 10 reactions</p>

cellscript Modified NTP In Vitro Transcription C-ICTMY110510</p>

cellscript总代理 Modified NTP In Vitro Transcription C-ICTMY110510</p>

cellscript总代理中北林格 Modified NTP In Vitro Transcription C-ICTMY110510</p>


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