ScriptCap™ Cap 1 Capping System C-SCCS1710
产品简介:北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710,中北林格是cellscript总代理,提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理,epicentre总代理,lucigen总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>
ScriptCap™ Cap 1 Capping System C-SCCS1710 规格详情
名称:ScriptCap™ Cap 1 Capping System</p>
分类:Post-Transcriptional Capping</p>
货号:C-SCCS1710</p>
规格:10 reactions</p>
产品详情:For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.</p>
供应商:中北林格</p>产地:us</p>发货地:北京</p>运输方式:送货上门/顺丰</p>是否现货:期货</p>
ScriptCap™ Cap 1 Capping System C-SCCS1710 产品详情
A. Synthesis of Cap 0 RNA from Uncapped RNA
1. The protocol below was designed to build a cap 1 structure on 5' end of 50-60 µg of uncapped RNA. If a cap 0 structure is desired, replace the ScriptCap 2'-O-Methyltransferase in Step 3 with an equivalent volume of RNase-Free Water. Combine the following reagents:
Standard ScriptCap Cap 1 Capping Reaction (step 1)
|
Component
|
Amount
|
RNase-Free Water
|
x µl
|
In vitro transcribed RNA, 50-60 µg
|
≤67 µ
|
Total Volume
|
67 µl
|
2. Incubate at 65o C for 5-10 minutes, then transfer to ice.
3. While the heat-denatured RNA is cooling on ice, prepare a “Cocktail” of the following reaction components together in a separate tube.
Standard ScriptCap Cap 1 Capping Reaction (step 3)
|
Component
|
Amount
|
10X ScriptCap Capping Buffer
|
10 µl
|
10 mM GTP
|
10 µl
|
20 mM SAM
|
2.5 µl
|
ScriptGuard RNase Inhibitor
|
2.5 µl
|
ScriptCap 2'-O-Methyltransferase (100 U/µl)
|
4 µl
|
Total Volume
|
29 µl
|
4. Just prior to starting the reaction, add the ScriptCap Capping Enzyme to the Cocktail from Step 3 and then combine this with the uncapped RNA solution from Step 1.
Standard ScriptCap Cap 1 Capping Reaction (step 4)
|
Component
|
Amount
|
Cocktailed reaction components (from step 3)
|
29 µl
|
ScriptCap Capping Enzyme (10 U/µl)
|
4 µl
|
Heat-denatured RNA (from step 1)
|
67 µl
|
Total Reaction Volume
|
100 µl
|
5. Incubate at 37o C for 30 minutes.
6. The Capped RNA can now be purified, or it can be 3' polyadenylated without purification by adding the reaction mixture directly to an A-Plus Poly(A) Polymerase Tailing Kit reaction (sold separately).
ScriptCap™ Cap 1 Capping System Contents (10 Reactions)
|
Kit Component
|
Reagent Volume
|
ScriptCap™ Capping Enzyme, 10 U/µl (red cap)
|
40 µl
|
ScriptCap™ 2'-O-Methyltransferase, 100 U/µl (blue cap)
|
40 µl
|
10X ScriptCap™ Capping Buffer. 0.5 M Tris-HCl, pH 8.0, 60 mM KCl and 12.5 mM MgCl2
|
100 µl
|
10 mM GTP Solution
|
100 µl
|
20 mM S-adenosyl-methionine (SAM)
|
25 µl
|
ScriptGuard™ RNase Inhibitor, 40 U/µl
|
25 µl
|
RNase-Free Water
|
1.4 ml
|
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ScriptCap™ Cap 1 Capping System C-SCCS1710 关键词:
ScriptCap™ Cap 1 Capping System</p>
cellscript ScriptCap™ Cap 1 Capping System</p>
cellscript总代理 ScriptCap™ Cap 1 Capping System</p>
cellscript总代理中北林格 ScriptCap™ Cap 1 Capping System</p>
cellscript C-SCCS1710 10 reactions</p>
cellscript��代理 C-SCCS1710 10 reactions</p>
cellscript总代理中北林格 C-SCCS1710 10 reactions</p>
cellscript Post-Transcriptional Capping C-SCCS1710</p>
cellscript总代理 Post-Transcriptional Capping C-SCCS1710</p>
cellscript总代理中北林格 Post-Transcriptional Capping C-SCCS1710</p>
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