ScriptSeq™ Complete Kit (Human/Mouse/Rat) Low Input

ScriptSeq™ Complete Kits (人/大鼠/小鼠)RNA测序文库制备试剂盒(人/大鼠/小鼠)

应用说明

• 从少至100ng的总RNA制备测序文库，可用于Illumina公司®测序平台的使用。

The ScriptSeq™ Complete Kit 试剂盒结合了EPI公司Ribo-Zero™技术和ScriptSeq v2 RNA快速测序文库制备试剂盒的无缝末端连接技术，可在1天内实现RNA测序文库的制备（图1）。The ScriptSeq Complete Kits需要1-5微克的RNA,the ScriptSeq™ Complete Kits—Low Input 需要100ng-1ug的总RNA.
The ScriptSeq Complete Kit 包括 Ribo-Zero™试剂可去除细胞质（核编码）的rRNA，还包括零核糖金装试剂，可去除细胞质和线粒体核糖体RNA（图2）

特点说明：

• 实现完整的单日RNA测序文库制备。
• 产生方向性的RNA测序文库, 通常含<3％的rRNA读长序列。
• 使用低至100 ng的总RNA，包括FFPE RNA。
• 条码/标签兼容：ScriptSeq指数引物可单独编制有条形码库。

图1ScriptSeq™ Complete Kits过程概述。

可在1天时间内完成从低至100 ng的总RNA，包括FFPE RNA，的高通量测序RNA文库制备，几乎不含有rRNA.

图2 使用the ScriptSeq™ Complete Kit (Human/Mouse/Rat)—Low Input 和the ScriptSeq Complete Gold Kit (Human/Mouse/Rat)—Low Input. 从FFPE RNA 样品中制备的RNA测序文库评价。The ScriptSeq Complete Kit 包括 Ribo-Zero™试剂可去除细胞质（核编码）的rRNA，还包括零核糖金装试剂，可去除细胞质和线粒体核糖体RNA

ScriptSeq™ Complete Kit (Human/Mouse/Rat) Low Input

ScriptSeq™ Complete Kit Low Input SCL24H

产品清单说明

货号			规格

ScriptSeq™ Complete Kit (Human/Mouse/Rat)

BHMR1205	6 Reactions
BHMR1224	24 Reactions

Note: For use with 1-5 µg total RNA.
包含：Ribo-Zero™ rRNA Removal Reagents (Human/Mouse Rat), Magnetic Core Kit, ScriptSeq™ v2 RNA-Seq Library Preparation Kit.三部分

ScriptSeq™ Complete Gold Kit (Human/Mouse/Rat)

BG1206	6 Reactions
BG1224	24 Reactions

Note: For use with 1-5 µg total RNA. Contents: One box of Ribo-Zero™ Gold rRNA Removal Reagents (Human/Mouse Rat), one box of Magnetic Core Kit, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.

ScriptSeq™ Complete Kit (Human/Mouse/Rat)—Low Input

SCL24H	24 Reactions
SCL6H	6 Reactions

Note: For use with 100 ng to 1 µg total RNA. Contents: One box of Ribo-Zero™ rRNA Removal Reagents (Human/Mouse Rat)—Low Input, one box of Magnetic Core Kit—Low Input, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.

ScriptSeq™ Complete Gold Kit (Human/Mouse/Rat)—Low Input

SCL24G	24 Reactions
SCL6G	6 Reactions

Note: For use with 100 ng to 1 µg total RNA. Contents: One box of Ribo-Zero™ Gold rRNA Removal Reagents (Human/Mouse/Rat)—Low Input, one box of Magnetic Core Kit—Low Input, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.

FailSafe™ PCR Enzyme Mix

FSE51100

100 Units

The FailSafe™ PCR Enzyme Mix is required for library preparation.

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