MonsterScript™ Reverse Transcriptase MSTA5110

• First-strand cDNA synthesis for subsequent PCR or real-time PCR.
• RT-PCR validation of gene expression data obtained from microarray experiments.
• RT-PCR validation and quantification of gene silencing by RNA interference.
• Preparation of labeled cDNA probes.
• 用于后续PCR或实时PCR的第1链cDNA合成。
• 从微阵列实验获得的基因表达数据的RT-PCR验证。
• RT-PCR验证和RNA干扰对基因沉默的定量。
• 标记的cDNA探针的制备。

MonsterScript™ Reverse Transcriptase is a thermostable reverse transcriptase that completely lacks RNase H activity. The enzyme's lack of RNase H activity contributes to its ability to make longer cDNAs and more complete full-length libraries of first-strand cDNA molecules compared to other reverse transcriptases. EPICENTRE scientists have demonstrated reverse transcription of 15-kb RNA templates using MonsterScript™ Reverse Transcriptase (Fig. 1).

MonsterScript™逆转录酶是完全缺乏RNase H活性的热稳定逆转录酶。与其他逆转录酶相比，该酶缺乏RNase H活性有助于其制备更长的cDNA和更完整的第1链cDNA分子全长文库的能力。EPICENTER科学家利用MonsterScript™逆转录酶证明了15-kb RNA模板的逆转录作用（图1）。

The MonsterScript 1st-Strand cDNA Synthesis Kit includes MonsterScript RT PreMix, which contains optimized concentrations of dNTPs, Mg²⁺, and betaine.* Betaine reduces pausing and stops by the reverse transcriptase, enabling improved reverse transcription through difficult sequences, such as regions of high GC content. The kit also contains both an oligo(dT)-containing primer and random nonamer primers.

MonsterScript 1st-Strand cDNA Synthesis Kit包含MonsterScript RT PreMix，其中包含优化浓度的dNTPs，Mg ²⁺和甜菜碱。*甜菜碱可减少逆转录酶的停顿和终止，从而通过困难的序列（例如， GC含量高。该试剂盒还包含含寡聚（dT）的引物和随机九聚体引物。

Benefits

• No RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming.
• Thermostable, permitting reverse transcription at temperatures >50°C, which reduces RNA secondary structure and improves priming specificity.
• MonsterScript RT PreMix contains optimized concentrations of dNTPs, Mg²⁺, and betaine for superior performance and minimal pipetting steps.
• The kit includes both an oligo(dT) and random nonamer primers for maximal transcript coverage.
• First-strand cDNA can be made from picogram amounts of total RNA (Fig. 2).
• The kit includes a potent RNase Inhibitor to protect the integrity of template RNA.

• 没有RNase H活性，即使使用长引物，也可以使用随机引物改善全长cDNA的合成。
• 具有热稳定性，可在> 50°C的温度下进行逆转录，从而减少RNA二级结构并提高引物特异性。
• MonsterScript RT PreMix包含优化浓度的dNTPs，Mg ²⁺和甜菜碱，以提供**的性能和zui少的移液步骤。
• 该试剂盒包括寡核苷酸（dT）和随机九聚体引物，可zui大程度地覆盖转录本。
• 第1链cDNA可以由皮克总量的总RNA制成（图2）。
• 该试剂盒包含有效的RNase抑制剂，可保护模板RNA的完整性。