1X DNase Buffer lucigen epicentre MBD092H

• Purification of TNA, DNA, or RNA for many applications, including PCR, RT-PCR, cloning, sequencing, Northern and Southern blotting, restriction analysis, and genomic library preparation.
• TNA，DNA或RNA的纯化可用于许多应用，包括PCR，RT-PCR，克隆，测序，Northern和Southern杂交，限制性分析和基因组文库制备。

The MasterPure™ Complete DNA and RNA Purification Kit uses a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table 1 and Fig. 1).

MasterPure™完整的DNA和RNA纯化试剂盒采用了一种新颖的技术，可以从每种类型的生物材料中有效纯化完整的总核酸（TNA），DNA或RNA（表1和图1）。

The gentle salt-precipitation protocol permits rapid purification of high-molecular-weight nucleic acids from a single sample or many samples that are processed simultaneously (Fig. 4). High yields of nucleic acids are obtained using MasterPure Kits (Table 2), and yields and purity are highly consistent for different samples of the same material (Fig. 2). The resultant nucleic acids have an A₂₆₀/A₂₈₀ ratio of 1.8-2.0, indicating that they are substantially free of protein. The TNA, DNA, and/or RNA purified from different samples can be used directly in many applications. For example, the sensitivity of PCR or RT-PCR assays is often improved if the template is obtained using a MasterPure Purification Kit.

温和的盐沉淀方案允许从单个样品或同时处理的多个样品中快速纯化高分子量核酸（图4）。使用MasterPure试剂盒可获得高产量的核酸（表2），并且同一材料的不同样品的产量和纯度高度一致（图2）。所得核酸的A ₂₆₀ / A ₂₈₀  比为1.8-2.0，表明它们基本上不含蛋白质。从不同样品中纯化的TNA，DNA和/或RNA可以直接用于许多应用中。例如，如果使用MasterPure纯化试剂盒获得模板，则通常会提高PCR或RT-PCR分析的灵敏度。

1X DNase Buffer lucigen epicentre MBD092H   优势说明

• Purify TNA in 30 minutes without cumbersome columns.
• 在30分钟内纯化TNA，无需繁琐的色谱柱。
• A₂₆₀/A₂₈₀ ratios consistently between 1.8 and 2.0.
• 阿₂₆₀ / A _280个  比例一致1.8和2.0之间。
• Recover >90% of theoretical DNA yield.
• 回收> 90％的理论DNA产量。
• No phenol, chloroform, or other caustic solvents.
• 没有苯酚，氯仿或其他苛性溶剂。
• Each kit component is also available separately in a 10X package size.
• 每个套件组件也都单独提供10倍的包装尺寸。
• Low- and high-molecular-weight nucleic acids are recovered.
• 回收低分子量和高分子量核酸。

Reference

1. Schanke, J. and Watson, J. (1998) EPICENTRE Forum 5(2), 12.