epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit
产品简介：北京中北林格供应epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit，中北林格是epicentre总代理，提供epicentre产品货号报价查询。其他包括lucigen总代理，cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
产品名称:MasterAmp™ High Fidelity RT-PCR Kit
货号:RF91025
规格:25 Rxns
存储温度:-20℃
品牌：lucigen / epicentre</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit 产品描述
Applications
-
Highly accurate RT-PCR amplification of RNA templates for cloning, sequencing, expression, and transcription analysis.
-
All-purpose RT-PCR in which full-length amplification and high accuracy are essential.
-
Multiplex RT-PCR.
-
RNA模板的高精度RT-PCR扩增，可用于克隆，测序，表达和转录分析。
-
通用RT-PCR，其中全长扩增和高精度至关重要。
-
多重RT-PCR。
The MasterAmp™ High Fidelity RT-PCR Kit is ideal for synthesis of accurate double-stranded cDNA products that will be used for cloning, sequencing, expression, or transcription analysis. The kit uses MMLV-RT Plus, a reverse transcriptase with an unsurpassed accuracy and reliability, and MasterAmp TAQurate™ DNA Polymerase, a high-fidelity PCR enzyme mix (Fig. 1). The kit also includes an optimized 2X RT-PCR Buffer PreMix (containing dNTPs, MgCl2, and buffer), random and oligo(dT) primers, and the MasterAmp PCR Enhancer with betaine,* which can substantially improve the yield and specificity of RT-PCR amplification.
MasterAmp™高保真RT-PCR试剂盒非常适合合成精que的双链cDNA产品，该产品将用于克隆，测序，表达或转录分析。该试剂盒使用MMLV-RT Plus（一种具有****的准确性和可靠性的逆转录酶）和MasterAmp TAQurate™DNA聚合酶（一种高保真度PCR酶混合物）（图1）。该试剂盒还包括优化的2X RT-PCR缓冲液PreMix（包含dNTP，MgCl 2和缓冲液），随机和oligo（dT）引物，以及具有甜菜碱的MasterAmp PCR增强剂*，可大大提高RT的产量和特异性。 -PCR扩增。
Benefits
-
High-fidelity RT-PCR (i.e., low error rates).
-
Amplification of full-length products from RNA templates up to ~6 kb (Fig. 2).
-
Random and oligo(dT) primers included for first-strand cDNA synthesis.
-
Unique PreMix reduces pipetting steps and chances of contamination.
-
Uses MasterAmp PCR Enhancer for better amplification of high-GC or other difficult templates.
-
高保真RT-PCR（即低错误率）。
-
从RNA模板扩增全长产物，zui大约6 kb（图2）。
-
包括随机和oligo（dT）引物，用于第1链cDNA合成。
-
独特的PreMix减少了移液步骤和污染机会。
-
使用MasterAmp PCR增强剂可更好地扩增高GC或其他困难的模板。
epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit 图例说明
Figure 1. Different RT-PCR protocols that can be used with the MasterAmp™ High Fidelity RT-PCR Kit.
图1.可以与MasterAmp™高保真RT-PCR试剂盒一起使用的不同RT-PCR方案。
Figure 2. The MasterAmp™ High Fidelity RT-PCR Kit amplifies full-length products from RNA templates up to ~6 kb. RT-PCR products from Tobacco Mosaic Virus RNA with primers that amplify a 5.5-kb region of the viral RNA. Lane M, Kilobase ladder; lane 1, RT-PCR using the MasterAmp one-step protocol; lane 2, RT-PCR using MMLV-RT and Taq DNA polymerase with a standard two-step procedure; lane 3, RT-PCR using TthDNA polymerase.
图2. MasterAmp™高保真RT-PCR试剂盒可扩增RNA模板中的全长产物，zui大约6 kb。**花叶病毒RNA的RT-PCR产物，带有引物，可扩增病毒RNA的5.5-kb区域。M线，千磅梯；泳道1，使用MasterAmp一步协议进行RT-PCR；泳道2，使用MMLV-RT和Taq DNA聚合酶的RT-PCR，采用标准的两步操作；泳道3，使用Tth DNA聚合酶的RT-PCR 。
Figure 3. Amplification of a variety of RNA templates using a one-step protocol with the MasterAmp™ High Fidelity RT-PCR Kit. Lane M, 100-bp ladder; lane 1, human lung β-actin; lane 2, human lung GAPDH; lane 3, region of TMV; lane 4, E. coli 16S rRNA; lane 5, human CGα; lane 6, human heart β-actin; lane 7, region of TMV.
图3.使用MasterAmp™高保真RT-PCR试剂盒一步一步扩增各种RNA模板。 M道，100 bp阶梯; 泳道1，人肺β-肌动蛋白；泳道2，人肺GAPDH；TMV区域3泳道；泳道4， 大肠杆菌 16S rRNA；人CGα5泳道；泳道6，人的心脏β-肌动蛋白；TMV的第7车道。
Covered by issued and/or pending patents.
MasterAmp™ High Fidelity RT-PCR Kit. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
对此产品有兴趣，可联系中北林格获取更多详细信息。</p>
- 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
- 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报