Applications
HK™-UNG is a Uracil N-Glycosylase that can be easily heat-inactivated. Uracil N-Glycosylase (also known as uracil-DNA glycosylase) hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. HK-UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. HK-UNG is ideal for studying repair of abasic sites in double-stranded DNA.
HK™-UNG是一种尿嘧啶N-糖基化酶，可以很容易地热灭活。尿嘧啶Ñ -Glycosylase（也称为尿嘧啶-DNA糖基化酶）水解脱氧核糖和尿嘧啶在含有代替胸苷的脱氧尿苷DNA之间的N-糖苷键。HK-UNG对含有尿嘧啶的ssDNA和dsDNA均具有活性，但对RNA或2´-deoxyuridine-5´-monophosphate却没有活性。HK-UNG是研究双链DNA无碱基位点修复的理想选择。
Unit Definition: One unit of HK-UNG catalyzes the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37°C under standard assay conditions.
单位定义：在标准测定条件下，于37°C在1小时内，HK-UNG的一个单位可在1小时内从含尿嘧啶的DNA释放1 nmol尿嘧啶。
Note: EPICENTRE's unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.
注意： EPICENTRE的设备比其他供应商定义的设备活跃5到20倍。因此，提供了稀释缓冲液用于需要较低浓度酶的应用。
Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
稀释和储存缓冲液： 50％甘油，其中含有50 mM Tris-HCl（pH 7.5），100 mM NaCl，0.1 mM EDTA，1 mM DTT和0.1％Triton®X-100。
Quality Control: HK™-UNG is free of detectable exo- and endonuclease and RNase activities.
质量控制： HK™-UNG不含可检测的外切核酸酶和内切核酸酶以及RNase活性。
epicentre HU59100 HK™-UNG Thermolabile Uracil N-Glycosylase 图例说明