PlasmidMAX™DNA分离试剂盒采用改良的碱裂解程序,无需使用色谱柱或有害化学物质即可纯化不含RNA和基因组DNA的质粒。这个简单的过程只需要两个1.5 ml试管,从而避免了多余的塑料浪费。纯化方法可扩展至更大体积,因此不需要单独的试剂盒即可改变样品大小。对于3-40 kb的质粒,从使用PlasmidMAX试剂盒分离的DNA获得的平均序列读数> 600个碱基。 The PlasmidMAX™ DNA Isolation Kit purifies plasmids free from RNA and genomic DNA without the use of columns or hazardous chemicals, using a modified alkaline-lysis procedure. This simple procedure requires only two 1.5-ml tubes, thus avoiding excess plastic waste. The purification method is scaleable to larger volumes, so separate kits are not needed for varying sample sizes. For plasmids 3-40 kb, average sequence reads of >600 bases are obtained from DNA isolated using the PlasmidMAX Kit.
PlasmidMAX试剂盒处理
1.造粒细胞。重悬于解决方案1。
2.添加溶液2.混合。
3.添加溶液3.混合。
4.颗粒细胞碎片和染色体DNA。
5.异丙醇沉淀核酸。
6.用RiboShredder™RNase消化RNA。
7.沉淀并洗涤质粒DNA。
1. Pellet cells. Resuspend in Solution 1.
2. Add Solution 2. Mix.
3. Add Solution 3. Mix.
4. Pellet cell debris and chromosomal DNA.
5. Isopropanol precipitate nucleic acid.
6. Digest RNA with RiboShredder™ RNase.
7. Precipitate and wash plasmid DNA.