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epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian)

epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian)
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简单介绍
北京中北林格供应epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian),中北林格是epicentre总代理,提供epicentre产品货号报价查询。其他包括lucigen总代理,cellscript总代理,Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian)
产品描述
epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian)
产品简介:北京中北林格供应epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian),中北林格是epicentre总代理,提供epicentre产品货号报价查询。其他包括lucigen总代理,cellscript总代理,Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p

产品名称:ARTSeq Ribosome Profiling Kit (Mammalian)
货号:RPHMR12116
规格:12 Rxns
存储温度:-70° C
品牌:lucigen / epicentre</p>
供应商:中北林格</p>
产地:us</p>
发货地:北京</p

epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian) 产品描述

ARTseq™核糖体分析试剂盒

 

应用领域

ARTseq™试剂盒从核糖体保护的mRNA产生RNA-Seq文库,以:

The ARTseq™ Kit produces RNA-Seq libraries from ribosome-protected mRNA to:

  • Investigate translational control.
  • 研究翻译控制。
  • Measure gene expression.
  • 测量基因表达。
  • Identify translation start sites.
  • 确定翻译开始站点。
  • Predict protein abundance.
  • 预测蛋白质丰度。
  • Investigate translational and co-translational processes in vivo.
  • 研究体内翻译和共翻译过程

核糖体分析是研究翻译控制的一种新技术。该方法基于核糖体保护的mRNA片段的深度测序,提供了在特定时间点在细胞中活跃的核糖体的“快照”。该信息又可以用于确定细胞中正在主动翻译哪些蛋白质。

Ribosome profiling is a novel technique for investigating translational control. The method, based on deep sequencing of ribosome protected mRNA fragments, provides a "snapshot" of the ribosomes active in a cell at a specific time point. This information can in turn be used to determine what proteins are being actively translated in a cell.

优势说明

  • 合并了一种快速且简化的尺寸排阻旋转柱方法来分离单核细胞。
  • Incorporates a rapid and simplified size-exclusion spin-column method to isolate monsomes.
  • 与酵母和哺乳动物样品兼容。
  • Compatible with yeast and mammalian samples.
  • ARTseq库与Illumina®簇试剂盒兼容,可使用Illumina GAII,HiSeq和MiSeq测序仪进行单读,配对末端和多重测序。
  • ARTseq libraries are compatible with Illumina® Cluster Kits for single read, paired-end and multiplex sequencing using the Illumina GAII, HiSeq and MiSeq sequencers.
  • 兼容索引:套件中包含12种Index Primers。
  • Index-compatible: 12 Index Primers included in the kit.

epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian) 图例说明


图1(单击放大)。用于将核糖体保护片段(RPF)转换为RNA-Seq文库的ARTseq核糖体分析过程概述。 用环己酰亚胺(由用户提供)处理哺乳动物或酵母细胞,以维持mRNA的体内核糖体位置。核糖体足迹是通过用核糖核酸酶消化细胞裂解液并通过超速离心或尺寸排阻色谱法回收多核糖体或单核糖体而产生的。纯化后,将核酸酶保护的mRNA片段转换为DNA库,以便在任何Illumina®测序平台上进行测序。
Figure 1 (click to enlarge). Overview of the ARTseq Ribosome Profiling process for converting ribosome protected fragments (RPF) into an RNA-Seq library. Mammalian or yeast cells are treated with cycloheximide (provided by the user) to maintain the in vivo ribosome positions on the mRNAs. Ribosome footprints are generated by digesting cellular lysate with ribonuclease and recovering polysomes or monosomes by ultracentrifugation or size-exclusion chromatography. Following purification, the nuclease protected mRNA fragments are converted into a DNA library for sequencing on any Illumina® sequencing platform.

图2.核糖体保护的RNA或poly(A)+ mRNA样品沿代表性酵母转录物(CWP2)的测序读数分布。A)在尺寸排阻色谱法(SEC)或蔗糖梯度离心法(Gradient)之后制备的ARTseq RNA-Seq文库导致读取密度的分布基本相同,并且富含开放阅读框(ORF),并且没有未翻译序列(UTR)。B)与转录物的编码,UTR,内含子和基因间区域对齐的读段百分比的图形表示。
Figure 2. Distribution of sequencing reads from ribosome protected RNA or poly(A)+ mRNA samples along a representative yeast transcript (CWP2). A) ARTseq RNA-Seq libraries prepared after Size-exclusion chromatography (SEC) or sucrose gradient centrifugation (Gradient) result in essentially identical distribution of read densities and are enriched for Open Reading Frame (ORF) and devoid of Untranslated sequences (UTR). B) Graphic representation of percent of reads that align to coding, UTR, intron and intergenic regions of the the transcript.

epicentre RPHMR12116 ARTSeq Ribosome Profiling Kit (Mammalian)  货号详情
ARTseq™核糖体分析试剂盒-酵母
RPYSC12116 12反应
 
ARTseq™核糖体分析试剂盒-哺乳动物
RPHMR12126 12反应
 
Ribo-Zero™磁性套件(人类/小鼠/大鼠)(用于ARTseq)
MRZH11124 24个反应
用于从ARTseq库中去除rRNA。
Ribo-Zero™金装套件(人类/小鼠/大鼠)(用于ARTseq)
MRZG12324 24个反应
MRZG126 6反应
用于从ARTseq库中去除rRNA。
Ribo-Zero™磁性金试剂盒(酵母)(用于ARTseq)
MRZY1324 24个反应
用于从ARTseq库中去除rRNA。

ARTseq™ Ribosome Profiling Kit - Yeast
RPYSC12116 12 Reactions
 
ARTseq™ Ribosome Profiling Kit - Mammalian
RPHMR12126 12 Reactions
 
Ribo-Zero™ Magnetic Kit (Human/Mouse/Rat) (for ARTseq)
MRZH11124 24 Reactions
For removing rRNA from ARTseq libraries.
Ribo-Zero™ Gold Kits (Human/Mouse/Rat) (for ARTseq)
MRZG12324 24 Reactions
MRZG126 6 Reactions
For removing rRNA from ARTseq libraries.
Ribo-Zero™ Magnetic Gold Kit (Yeast) (for ARTseq)
MRZY1324 24 Reactions
For removing rRNA from ARTseq libraries.

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