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LK08050 Fast-Link™ DNA Ligation and Screening Kit

LK08050 Fast-Link™ DNA Ligation and Screening Kit
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简单介绍
北京中北林格供应LK08050 Fast-Link™ DNA Ligation and Screening Kit ,中北林格是epicentre总代理,提供epicentre产品货号报价查询。其他包括lucigen总代理,cellscript总代理,Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。LK08050 Fast-Link™ DNA Ligation and Screening Kit
产品描述
产品简介:北京中北林格供应LK08050 Fast-Link™ DNA Ligation and Screening Kit ,中北林格是epicentre总代理,提供epicentre产品货号报价查询。其他包括lucigen总代理,cellscript总代理,Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p

产品名称:Fast-Link™ DNA Ligation and Screening Kit
               Fast-Link™DNA连接和筛选试剂盒
货号:LK08050
规格:50 Lig.
存储温度:-20℃

品牌:lucigen / epicentre</p>
供应商:中北林格</p>
产地:us</p>
发货地:北京</p

LK08050 Fast-Link™ DNA Ligation and Screening Kit   应用领域

  • 快速有效的DNA连接。
  • Rapid and efficient DNA ligation.
  • Rapid screening of clones for insert size.
  • 快速筛选克隆的插入片段大小。

Fast-Link™DNA连接和筛选试剂盒将流行的Fast-Link DNA连接试剂盒的快速DNA连接与Colony Fast-Screen™试剂盒提供的快速,灵敏的克隆筛选(大小筛选)结合在一起。快速链接DNA连接和筛选试剂盒将DNA连接,转化和筛选重组质粒所需的时间减少到24小时或更短。因此,该试剂盒非常适合常规和高通量克隆和筛选项目。

The Fast-Link™ DNA Ligation and Screening Kit combines the rapid DNA ligations of the popular Fast-Link DNA Ligation Kit with the rapid and sensitive screening of clones afforded by the Colony Fast-Screen™ Kit (Size Screen). The Fast-Link DNA Ligation and Screening Kit reduces the time required for DNA ligation, transformation, and screening of recombinant plasmids to 24 hours or less. Thus, the kit is ideal for both routine and high-throughput cloning and screening projects.

LK08050 Fast-Link™ DNA Ligation and Screening Kit    优势说明

  • 室温下在5分钟内进行内聚端连接。
  • Cohesive-end ligations in 5 minutes at room temperature.
  • 在室温下15分钟内平端连接。
  • Blunt-end ligations in 15 minutes at room temperature.
  • 在16°C下在1小时内用A突出端连接PCR产物。
  • Ligation of PCR products with A-overhangs in 1 hour at 16°C.
  • 结扎效率高。
  • High ligation efficiency.
  • 不需要在电穿孔之前对连接产物进行脱盐。
  • Desalting of ligation products prior to electroporation is not necessary.
  • 简单快速地筛选重组质粒(1小时),而无需培养物或进行限制性酶切。
  • Simple and fast (1-hour) screening of recombinant plasmids, without the need to grow cultures or perform restriction digests.

LK08050 Fast-Link™ DNA Ligation and Screening Kit   图例说明

图1.使用Fast-Link™DNA连接和筛选试剂盒进行重组筛选的结果。克隆使用具有粘性末端(A),平末端(B)和PCR产物(C)的插入片段进行的连接,并使用孔内裂解方案筛选白色菌落。预期的插入物+载体大小用箭头指示。

Figure 1. Results of recombinant screening using the Fast-Link™ DNA Ligation and Screening Kit. Ligations performed using inserts with cohesive ends (A), blunt ends (B), and PCR products (C) were cloned, and white colonies were screened using an in-well lysis protocol. The expected insert+vector sizes are indicated with arrows.


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