Applications
The Colony Fast-Screen™ Kit (Restriction Screen) provides an easy and rapid method to screen the size and orientation of cloned DNA by restriction digests, without overnight cultures or plasmid minipreps (Fig. 1). The Colony Fast-Screen Kit Restricti-Lyse™ solution lyses cells in an environment which is not inhibitory to restriction enzyme activity. Thus, the kit enables the use of any restriction enzyme for restriction analysis. E. coli recombinant colonies can be screened in 25 minutes using the standard protocol (Fig. 2), or in as little as 10 minutes with the accelerated protocol (Fig. 3).
Colony Fast-Screen™试剂盒(限制性酶切)提供了一种简便,快速的方法,可通过限制性酶切来筛选克隆的DNA的大小和方向,而无需过夜培养或质粒小量制备(图1)。Colony快速筛选试剂盒Restricti-Lyse™解决方案可在不抑制限制性酶活性的环境中裂解细胞。因此,该试剂盒可将任何限制酶用于限制分析。 可以使用标准方案在25分钟内筛选出大肠杆菌重组菌落(图2),或者使用加速方案在短短10分钟内筛选出大肠杆菌重组菌落(图3)。
Benefits
Notes: