Applications
Lambda Exonuclease is a highly processive 5´→3´ exodeoxyribonuclease that selectively digests the 5´ phosphorylated strand of dsDNA. The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA. The enzyme has reduced activity against nicked DNA and against ssDNA and gapped DNA.3
Lambda外切核酸酶是一种高度加工性的5´→3´外脱氧核糖核酸酶,可选择性消化dsDNA的5´磷酸化链。优选的底物是平末端的5'-磷酸化dsDNA。该酶对带切口的DNA以及对ssDNA和缺口DNA的活性降低。3
Unit Definition: One unit of Lambda Exonuclease will produce 10 nmol of acid-soluble deoxyribonucleotides from dsDNA template in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer.
单位定义:一个单位的Lambda核酸外切酶将在1X Lambda核酸外切酶反应缓冲液中于37°C在30分钟内从dsDNA模板产生10 nmol的酸溶性脱氧核糖核苷酸。
质量控制: Lambda核酸外切酶经过功能测试,以确保使用5′-磷酸化引物产生的PCR产物完全且优先降解。使用含有5´-OH末端的引物制备的PCR产物不会被消化。经测试,λ外切核酸酶不含污染性核酸内切酶活性。
Quality Control: Lambda Exonuclease is function-tested to ensure complete and preferential degradation of PCR products produced using 5´-phosphorylated primers. PCR products made using primers containing 5´-OH ends are not digested. Lambda Exonuclease is tested to be free of contaminating endonuclease activities.
References