epicentre PlndigoBAC-5 Hind BamH III-Cloning Ready) Vector

Linearized and dephosphorylated Cloning-ready BAC Vectors

线性化和去磷酸化的可克隆的BAC向量

pIndigoBAC-5 (Fig. 1) is derived from pBeloBAC11 and pIndigoBAC¹ and will accommodate and stably maintain DNA inserts of >100 kb. pIndigoBAC-5 has been linearized at its unique BamH I or Hind III site, dephosphorylated, and highly purified and is ready for cloning BamH I- or Hind III-cut genomic DNA. The linearized and dephosphorylated vectors are tested to ensure the completeness of linearization, dephosphorylation, and the integrity of the BamH I and Hind III ends. The complete sequence and restriction map of pIndigoBAC-5 is available.

pIndigoBAC-5（图1）源自pBeloBAC11和pIndigoBAC ¹，可容纳并稳定地保持大于100 kb的DNA插入片段。pIndigoBAC-5已在其独特的BamH I或Hin d III位点线性化，去磷酸化并高度纯化，可用于克隆Bam H I或Hin d III切割的基因组DNA。测试线性化和去磷酸化的载体以确保线性化，去磷酸化的完整性以及Bam H I和Hin d III末端的完整性。pIndigoBAC-5的完整序列和限制性图谱都可以使用。

Once DNA has been ligated into the pIndigoBAC-5 vector, BAC libraries can be produced using the high efficiency TransforMax™ EC100™ Electrocompetent E. coli or the Phage T1-resistant TransforMax™ EC100™-T1^R Electrocompetent E. coli. These cells accommodate and stably maintain large BAC clones. Sequencing primers for BAC end-sequencing of pIndigoBAC-5 clones are available separately.

一旦DNA被连接入pIndigoBAC-5载体，可以使用高效率TransforMax™EC100™电感受产生BAC文库的大肠杆菌或噬菌体T1耐TransforMax™EC100™-T1 ^ř电感受态大肠杆菌。这些细胞容纳并稳定维持大型BAC克隆。pIndigoBAC-5克隆的BAC末端测序的测序引物可单独获得。

Note: pIndigoBAC-5 clones cannot be induced to high-copy number. To construct a library of BAC clones that can be induced to high-copy number, see the CopyControl™ BAC Cloning Kits. Individual BamH I, EcoR I, or Hind III Cloning-Ready CopyControl pCC1BAC Vectors are also avialable separately.

注意：无法将pIndigoBAC-5克隆诱导为高拷贝数。要构建可诱导为高拷贝数的BAC克隆文库，请参见CopyControl™BAC克隆试剂盒。单独的Bam H I，Eco R I或Hin d III复制准备就绪的CopyControl pCC1BAC载体也可单独获得。

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